HEADER TRANSCRIPTION REGULATION 06-MAR-92 1D66 1D66 2 COMPND GAL4 (RESIDUES 1 - 65) COMPLEX WITH 19MER DNA 1D66 3 SOURCE (SACCHAROMYCES $CEREVISIAE) OVEREXPRESSED IN (ESCHERICHIA 1D66 4 SOURCE 2 $COLI) 1D66 5 AUTHOR R.MARMORSTEIN,S.HARRISON 1D66 6 REVDAT 1 15-APR-93 1D66 0 1D66 7 JRNL AUTH R.MARMORSTEIN,M.CAREY,M.PTASHNE,S.C.HARRISON 1D66 8 JRNL TITL /DNA$ RECOGNITION BY /GAL4$: STRUCTURE OF A 1D66 9 JRNL TITL 2 PROTEIN(SLASH)/DNA$ COMPLEX 1D66 10 JRNL REF NATURE V. 356 408 1992 1D66 11 JRNL REFN ASTM NATUAS UK ISSN 0028-0836 006 1D66 12 REMARK 1 1D66 13 REMARK 2 1D66 14 REMARK 2 RESOLUTION. 2.7 ANGSTROMS. 1D66 15 REMARK 3 1D66 16 REMARK 3 REFINEMENT. 1D66 17 REMARK 3 PROGRAM CORELS;TNT;XPLOR 1D66 18 REMARK 3 AUTHORS J.SUSSMAN;D.TRONRUD;A.BRUNGER 1D66 19 REMARK 3 R VALUE 0.230 1D66 20 REMARK 3 RMSD BOND DISTANCES 0.015 ANGSTROMS 1D66 21 REMARK 3 RMSD BOND ANGLES 2.9 DEGREES 1D66 22 REMARK 4 1D66 23 REMARK 4 THERE ARE TWO DNA CHAINS WHICH HAVE BEEN ASSIGNED CHAIN 1D66 24 REMARK 4 INDICATORS *D* AND *E*. THERE ARE TWO PROTEIN CHAINS 1D66 25 REMARK 4 WHICH HAVE BEEN ASSIGNED CHAIN INDICATORS *A* AND *B*. 1D66 26 REMARK 4 EACH PROTEIN - DNA COMPLEX CONTAINS FOUR BOUND CD IONS. 1D66 27 REMARK 5 1D66 28 REMARK 5 THE PROTEIN CONTAINS THE N-TERMINAL 65 RESIDUES OF GAL4 1D66 29 REMARK 5 PLUS A C-TERMINAL PHE DERIVED FROM THE CLONING CONSTRUCT. 1D66 30 REMARK 6 1D66 31 REMARK 6 RESIDUES LEU A 19 - LYS A 27 AND LEU B 19 - LYS B 27 FORM 1D66 32 REMARK 6 TIGHT TURNS WHICH CONNECT HELICES. RESIDUES TRP A 39 - 1D66 33 REMARK 6 LEU A 49 AND TRP B 39 - LEU B 49 FORM EXTENDED CHAINS 1D66 34 REMARK 6 WHICH CONNECT HELICES. 1D66 35 REMARK 7 1D66 36 REMARK 7 RESIDUES LYS A 18, ASN A 35, LYS B 18, AND ASN B 35 HAVE 1D66 37 REMARK 7 POSITIVE PHI ANGLES. 1D66 38 REMARK 8 1D66 39 REMARK 8 THE TRANSFORMATION PRESENTED ON *MTRIX* RECORDS BELOW WILL 1D66 40 REMARK 8 YIELD APPROXIMATE COORDINATES FOR CHAIN *B* WHEN APPLIED TO 1D66 41 REMARK 8 CHAIN *A*. 1D66 42 REMARK 9 1D66 43 REMARK 9 THE HYDROGEN BONDS BETWEEN BASE PAIRS IN THIS ENTRY FOLLOW 1D66 44 REMARK 9 THE CONVENTIONAL WATSON-CRICK HYDROGEN BONDING PATTERN. 1D66 45 REMARK 9 THEY HAVE NOT BEEN PRESENTED ON *CONECT* RECORDS IN THIS 1D66 46 REMARK 9 ENTRY. 1D66 47 SEQRES 1 D 19 C C G G A G G A C A G T C 1D66 48 SEQRES 2 D 19 C T C C G G 1D66 49 SEQRES 1 E 19 C C G G A G G A C T G T C 1D66 50 SEQRES 2 E 19 C T C C G G 1D66 51 SEQRES 1 A 66 MET LYS LEU LEU SER SER ILE GLU GLN ALA CYS ASP ILE 1D66 52 SEQRES 2 A 66 CYS ARG LEU LYS LYS LEU LYS CYS SER LYS GLU LYS PRO 1D66 53 SEQRES 3 A 66 LYS CYS ALA LYS CYS LEU LYS ASN ASN TRP GLU CYS ARG 1D66 54 SEQRES 4 A 66 TYR SER PRO LYS THR LYS ARG SER PRO LEU THR ARG ALA 1D66 55 SEQRES 5 A 66 HIS LEU THR GLU VAL GLU SER ARG LEU GLU ARG LEU GLU 1D66 56 SEQRES 6 A 66 PHE 1D66 57 SEQRES 1 B 66 MET LYS LEU LEU SER SER ILE GLU GLN ALA CYS ASP ILE 1D66 58 SEQRES 2 B 66 CYS ARG LEU LYS LYS LEU LYS CYS SER LYS GLU LYS PRO 1D66 59 SEQRES 3 B 66 LYS CYS ALA LYS CYS LEU LYS ASN ASN TRP GLU CYS ARG 1D66 60 SEQRES 4 B 66 TYR SER PRO LYS THR LYS ARG SER PRO LEU THR ARG ALA 1D66 61 SEQRES 5 B 66 HIS LEU THR GLU VAL GLU SER ARG LEU GLU ARG LEU GLU 1D66 62 SEQRES 6 B 66 PHE 1D66 63 FTNOTE 1 1D66 64 FTNOTE 1 RESIDUES PRO A 26 AND PRO B 26 ARE CIS-PROLINES. 1D66 65 HET CD 39 1 CADMIUM 1D66 66 HET CD 40 1 CADMIUM 1D66 67 HET CD 41 1 CADMIUM 1D66 68 HET CD 42 1 CADMIUM 1D66 69 FORMUL 5 CD 4(CD1) 1D66 70 FORMUL 6 HOH *51(H2 O1) 1D66 71 HELIX 1 H1A CYS A 11 LYS A 18 1 RESIDUE 18 HAS POSITIVE PHI 1D66 72 HELIX 2 H2A CYS A 28 ASN A 35 1 RESIDUE 35 HAS POSITIVE PHI 1D66 73 HELIX 3 H3A THR A 50 LEU A 64 1 1D66 74 HELIX 4 H1B CYS B 11 LYS B 18 1 RESIDUE 18 HAS POSITIVE PHI 1D66 75 HELIX 5 H2B CYS B 28 ASN B 35 1 RESIDUE 35 HAS POSITIVE PHI 1D66 76 HELIX 6 H3B THR B 50 LEU B 64 1 1D66 77 CRYST1 80.850 80.850 73.700 90.00 90.00 90.00 P 43 21 2 8 1D66 78 ORIGX1 1.000000 0.000000 0.000000 0.00000 1D66 79 ORIGX2 0.000000 1.000000 0.000000 0.00000 1D66 80 ORIGX3 0.000000 0.000000 1.000000 0.00000 1D66 81 SCALE1 0.012369 0.000000 0.000000 0.00000 1D66 82 SCALE2 0.000000 0.012369 0.000000 0.00000 1D66 83 SCALE3 0.000000 0.000000 0.013569 0.00000 1D66 84 MTRIX1 1 0.969990 0.014680 -0.242700 7.19246 1 1D66 85 MTRIX2 1 0.014290 -0.999900 -0.003900 83.38941 1 1D66 86 MTRIX3 1 -0.242710 -0.000190 -0.970100 62.87497 1 1D66 87